It offers single plex, multiplex, multianalyte and customizable possibilities. Fluorchem fc3 manual lens upgrade for alphaimager hp for chemiluminescence applications. Safety datasheets product inserts user guides service contracts ice cfr software. Proteinsimple protein detection instrument maurice manual. Proteinsimple maurice protein detection instrument user manual maurice manual. Both ice280 and ice3 use protein absorption at 280 nm to monitor charge heterogeneity, and now maurice adds native fluorescence detection to greatly increase cief capabilities. Variability in the manual processes that used to impact reproducibility, quantitation, time to result and overall data reliability is eliminated.
Mfi bot1 autosampler reagents and consumables are designed for use on the bot1 autosampler when it is attached to the mfi 5100 or mfi 5200 instrument. User manuals, icematic ice maker operating guides and service manuals. Cartridge installation and removal on ice3 proteinsimple. Select the ice function under digital ice 3 from the toolbar menu at the top of the cyberview x screen to enable or disable the ice function. Jess gives you four different ways to analyze proteins. Best of all, the transition is simple the same antibodies you use for western blotting protocols can be used with simple western assays. Maurice, ice3, and ice280 data equivalency for cief charge heterogeneity absorbance assays. Proteinsimple ice280 first wholecolumn cief system by proteinsimple. Our goal is to make protein analysis simpler, more quantitative and affordable. Proteinsimple polypropylene vial with 300ul manufacturer. Mfi 5200 particle analyzer from proteinsimple biocompare. Use the email address on your account instead of your user id to sign in with your current password. We develop and commercialise proprietary systems and consumables for protein analysis that ultimately help reveal new insights into the true nature of proteins.
Changing sample protein identification page 3 a check mark will appear next to the injection in the experiment pane to indicate a manual correction was made. Scott mack is a senior development scientist with proteinsimple currently supporting analysts utilizing imaged capillary electrophoresis ice, simple western and microflow imaging mfi technology for the characterization of protein therapeutics. Simple plex which biomarkers are present and how much. Biorad gs800 calibrated densitometer user manual calibration tablet areascalibration windows the gs800 densitometer is equipped with reflectance and transmission step wedges. Japanese using the simple western total protein assay to normalize immunoassay data in the same run. User manual instruction guide for proteinsimple devices proteinsimple user manuals proteinsimple. Simple western which protein is present and how much. Click roc function under digital ice3 menu, roc setting window will open up. Before you do anything else, please take a look in the box and check you have the items shown below. The maurice cief method development kit is a one stop. User manual instruction guides for proteinsimple devices. Regulatory agencies routinely require assessment of charge heterogeneity for biotechnology products. This user s guide demystifies protein and amino acids and explains how readers can use them to enhance their health.
As an added bonus, you can use the same generic method for multiple moleculesno need for productspecific methods. For information on flow cell options, please refer to the mfi 5000 series reagents and consumables section. Maurice systems take cief up a notch, and cesds application is a breeze. The ice3 system performs free solution ief in a capillary column cief and detects focused protein zones using a whole column uv absorption detector that avoids disturbing these focused zones. The simple western is a western with none of the hassle. Fluorchem fc3 motorized lens upgrade for alphaimager hp for chemiluminescence applications. Protein ice blue raspberry 12 drinks by ansi nutrition. Secrets of ice method design for protein therapeutics. User manual instruction guide for protein detection instrument maurice proteinsimple.
Protein simple ice3 user manual keyword found websites. Handset description handset description front view back view earpiece mirror camera speaker 65,000 colour main display left soft key right soft key. Proteinsimple 0453 polypropylene vial with 300ul molded insert, 100pkg. Now, with the latest compass software, researchers can easily validate their simple western work to ensure absolute data integrity. Protein simple ice3 user manual keyword after analyzing the system lists the list of keywords related and the list of websites with related content, in addition you can see which keywords most interested customers on the this website. Suitable for use with the ice3 alcott 720 and ice280alcott 719 only. Capillary isoelectric focusing of a difficulttodenature.
Ella helps the user to get fast and highly reproducible validated assay data with no manual steps. The ice280 was the first to do freesolution ief in a capillary column cief, detect focused protein zones using a whole column uv absorption detector, and not disturb those f. Maurice, ice3, and ice280 data equivalency for cief charge heterogeneity. Icematic ice maker user manuals download manualslib. To support the use of ice3 in qc and gmp environments, all required functionality is integrated in ice cfr software to ensure compliance with 21 cfr part 11. Capillary isoelectric focusing cief 1 is a useful technique for the determination of protein isoelectric point pi. With the pa 800 plus pharmaceutical analysis system, you can confidently safeguard the success of your biologics. Setup instructions, pairing guide, and how to reset. Get better overall workflow and time to result with ice3 and maurice theyre fluent in cief and cesds.
Biotechne warrants that the instrument purchased from biotechne the instrument will be free from defects in materials and workmanship during the applicable warranty period, provided that the instrument has been operated at all times in accordance with the instruction manual and user guide by or under the direct supervision of a certified. Proteinsimple ice280 ice systems first wholecolumn. Protein charge heterogeneity analysis in 10 minutes. Maurice protein detection instrument user manual maurice.
The maurice cief method development kit is a onestop. Simply load up to 96 samples, push a button, walk away and come back to fully analyzed data. System for analysis of protein heterogeneity protein simple ice3. Biologics ice3, maurice and mfi is my biologic drug pure. Proteinsimple maurice protein detection instrument user. Proteinsimple provides protein analysis tools to help researchers gain a better understanding of proteins and their role in disease. Perfect for all engineers seeking guidance on the principles of major civil engineering subject areas those in the first years of practice, the more experienced. Your data is reliable no matter whatacross samples, users, instruments or labs. Automatically restores original colors from the faded films. Characterization of the protein solubilizer simplesol for imaged capillary. Specialized in phosphoprotein signaling, protein purity, protein aggregation, protein fractionation, western blot imaging, gel documentation. Jess chemiluminescent and fluorescent western blotting. Faster and easier charge heterogeneity analysis with the ice 3.
Maurices native fluorescence detection for cief works by measuring the fluorescence emission of tryptophans aromatic group. Our goal is simply to help researchers gain a better understanding of proteins and their role in disease. The ice manuals series is the single most comprehensive collection of civil engineering knowledge available, covering key subject areas of civil engineering. Protein consists of amino acids, which are used in the construction of neurotransmitters, hormones, muscle and other tissues. Users guide to protein and amino acids basic health. Get better overall workflow and time to result with ice3 and maurice. This technology is unique in that it has the comparable resolution of traditional gel ief but incorporates the advantages.
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